treA Codifies for a Trehalase with Involvement in Xanthomonas citri subsp. citri Pathogenicity
Identifieur interne : 000004 ( Main/Exploration ); précédent : 000003; suivant : 000005treA Codifies for a Trehalase with Involvement in Xanthomonas citri subsp. citri Pathogenicity
Auteurs : André Vessoni Alexandrino ; Leandro Seiji Goto ; Maria Teresa Marques Novo-MansurSource :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Citrus canker, caused by the bacterium
Url:
DOI: 10.1371/journal.pone.0162886
PubMed: 27611974
PubMed Central: 5017680
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">treA Codifies for a Trehalase with Involvement in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> Pathogenicity</title><author><name sortKey="Alexandrino, Andre Vessoni" sort="Alexandrino, Andre Vessoni" uniqKey="Alexandrino A" first="André Vessoni" last="Alexandrino">André Vessoni Alexandrino</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author><author><name sortKey="Goto, Leandro Seiji" sort="Goto, Leandro Seiji" uniqKey="Goto L" first="Leandro Seiji" last="Goto">Leandro Seiji Goto</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author><author><name sortKey="Novo Mansur, Maria Teresa Marques" sort="Novo Mansur, Maria Teresa Marques" uniqKey="Novo Mansur M" first="Maria Teresa Marques" last="Novo-Mansur">Maria Teresa Marques Novo-Mansur</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27611974</idno><idno type="pmc">5017680</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017680</idno><idno type="RBID">PMC:5017680</idno><idno type="doi">10.1371/journal.pone.0162886</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">001097</idno><idno type="wicri:Area/Pmc/Curation">001096</idno><idno type="wicri:Area/Pmc/Checkpoint">000001</idno><idno type="wicri:Area/Ncbi/Merge">002166</idno><idno type="wicri:Area/Ncbi/Curation">002166</idno><idno type="wicri:Area/Ncbi/Checkpoint">002166</idno><idno type="wicri:Area/Main/Merge">000004</idno><idno type="wicri:Area/Main/Curation">000004</idno><idno type="wicri:Area/Main/Exploration">000004</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">treA Codifies for a Trehalase with Involvement in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> Pathogenicity</title><author><name sortKey="Alexandrino, Andre Vessoni" sort="Alexandrino, Andre Vessoni" uniqKey="Alexandrino A" first="André Vessoni" last="Alexandrino">André Vessoni Alexandrino</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author><author><name sortKey="Goto, Leandro Seiji" sort="Goto, Leandro Seiji" uniqKey="Goto L" first="Leandro Seiji" last="Goto">Leandro Seiji Goto</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author><author><name sortKey="Novo Mansur, Maria Teresa Marques" sort="Novo Mansur, Maria Teresa Marques" uniqKey="Novo Mansur M" first="Maria Teresa Marques" last="Novo-Mansur">Maria Teresa Marques Novo-Mansur</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Citrus canker, caused by the bacterium <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> (Xcc), is a severe disease of citrus. Xcc presents broad spectrum of citrus hosts including economically important species whereas <italic>X</italic>. <italic>fuscans</italic> subsp. <italic>aurantifolii–</italic>type C (XauC) causes a milder disease and only infects <italic>Citrus aurantifolia</italic>. Trehalase catalyzes hydrolysis of the disaccharide trehalose, a sugar that has been reported to be related to Xcc pathogenicity. We expressed the recombinant gene product and assessed Xcc trehalase structural and kinetics data. The recombinant protein presented 42.7% of secondary structures in α-helix and 13% in β-sheets, no quaternary structure in solution, and Michaelis-Menten constant (K<sub>M</sub>) of 0.077 mM and V<sub>max</sub> 55.308 μMol glucose.min<sup>-1</sup>.mg protein<sup>-1</sup> for trehalose. A Xcc mutant strain (XccΔtreA) was produced by gene deletion from Xcc genome. Enzymatic activity of trehalase was determined in Xcc, XauC and XccΔtreA cellular lysates, showing the highest values for XauC in <italic>in vitro</italic> infective condition and no activity for XccΔtreA. Finally, leaves of <italic>Citrus aurantifolia</italic> infected with XccΔtreA showed much more drenching and necrosis than those infected by wild type Xcc. We concluded that trehalase contributes to alleviate bacterial virulence and that inability for trehalose hydrolysis may promote higher Xcc infectivity.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gottwald, Tr" uniqKey="Gottwald T">TR Gottwald</name></author><author><name sortKey="Graham, Jh" uniqKey="Graham J">JH Graham</name></author><author><name sortKey="Schubert, Ts" uniqKey="Schubert T">TS Schubert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Civerolo, E" uniqKey="Civerolo E">E Civerolo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moreira, Lm" uniqKey="Moreira L">LM Moreira</name></author><author><name sortKey="Almeida, Nf" uniqKey="Almeida N">NF Almeida</name></author><author><name sortKey="Potnis, N" uniqKey="Potnis N">N Potnis</name></author><author><name sortKey="Digiampietri, La" uniqKey="Digiampietri L">LA Digiampietri</name></author><author><name sortKey="Adi, Ss" uniqKey="Adi S">SS Adi</name></author><author><name sortKey="Bortolossi, Jc" uniqKey="Bortolossi J">JC Bortolossi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Das, A" uniqKey="Das A">A Das</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Padmanabhan, D" uniqKey="Padmanabhan D">D Padmanabhan</name></author><author><name sortKey="Vidhyasekaran, P" uniqKey="Vidhyasekaran P">P Vidhyasekaran</name></author><author><name sortKey="Rajagopalan, C" uniqKey="Rajagopalan C">C Rajagopalan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goto, M" uniqKey="Goto M">M Goto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Da Silva, Ac" uniqKey="Da Silva A">AC da Silva</name></author><author><name sortKey="Ferro, Ja" uniqKey="Ferro J">JA Ferro</name></author><author><name sortKey="Reinach, Fc" uniqKey="Reinach F">FC Reinach</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author><author><name sortKey="Furlan, Lr" uniqKey="Furlan L">LR Furlan</name></author><author><name sortKey="Quaggio, Rb" uniqKey="Quaggio R">RB Quaggio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moreira, Lm" uniqKey="Moreira L">LM Moreira</name></author><author><name sortKey="De Souza, Rf" uniqKey="De Souza R">RF De Souza</name></author><author><name sortKey="Digiampietri, La" uniqKey="Digiampietri L">LA Digiampietri</name></author><author><name sortKey="Da Silva, Ac" uniqKey="Da Silva A">AC Da Silva</name></author><author><name sortKey="Setubal, Jc" uniqKey="Setubal J">JC Setubal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moreira, Lm" uniqKey="Moreira L">LM Moreira</name></author><author><name sortKey="Almeida, Nf" uniqKey="Almeida N">NF Almeida</name></author><author><name sortKey="Potnis, 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Chapman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elbein, Ad" uniqKey="Elbein A">AD Elbein</name></author><author><name sortKey="Pan, Yt" uniqKey="Pan Y">YT Pan</name></author><author><name sortKey="Pastuszak, I" uniqKey="Pastuszak I">I Pastuszak</name></author><author><name sortKey="Carroll, D" uniqKey="Carroll D">D Carroll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Strom, A" uniqKey="Strom A">A Strom</name></author><author><name sortKey="Kaasen, I" uniqKey="Kaasen I">I Kaasen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ausubel, Fm" uniqKey="Ausubel F">FM Ausubel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, Tn" uniqKey="Petersen T">TN Petersen</name></author><author><name sortKey="Brunak, S" uniqKey="Brunak S">S Brunak</name></author><author><name sortKey="Von Heijne, G" uniqKey="Von Heijne G">G von Heijne</name></author><author><name sortKey="Nielsen, H" uniqKey="Nielsen H">H Nielsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanger, F" uniqKey="Sanger F">F Sanger</name></author><author><name sortKey="Nicklen, S" uniqKey="Nicklen S">S Nicklen</name></author><author><name sortKey="Coulson, Ar" uniqKey="Coulson A">AR Coulson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sreerama, N" uniqKey="Sreerama N">N Sreerama</name></author><author><name sortKey="Woody, Rw" uniqKey="Woody R">RW Woody</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trinder, P" uniqKey="Trinder P">P Trinder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goto, Ls" uniqKey="Goto L">LS Goto</name></author><author><name sortKey="Alexandrino, Av" uniqKey="Alexandrino A">AV Alexandrino</name></author><author><name sortKey="Pereira, Cm" uniqKey="Pereira C">CM Pereira</name></author><author><name sortKey="Martins, Cs" uniqKey="Martins C">CS Martins</name></author><author><name sortKey="Pereira, Hdm" uniqKey="Pereira H">HDM Pereira</name></author><author><name sortKey="Brandao Neto, J" uniqKey="Brandao Neto J">J Brandão-Neto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ferreira, H" uniqKey="Ferreira H">H Ferreira</name></author><author><name sortKey="Barrientos, F" uniqKey="Barrientos F">F Barrientos</name></author><author><name sortKey="Baldini, R" uniqKey="Baldini R">R Baldini</name></author><author><name sortKey="Rosato, Y" uniqKey="Rosato Y">Y Rosato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bramucci, Mg" uniqKey="Bramucci M">MG Bramucci</name></author><author><name sortKey="Nagarajan, V" uniqKey="Nagarajan V">V Nagarajan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gibson, Rp" uniqKey="Gibson R">RP Gibson</name></author><author><name sortKey="Gloster, Tm" uniqKey="Gloster T">TM Gloster</name></author><author><name sortKey="Roberts, S" uniqKey="Roberts S">S Roberts</name></author><author><name sortKey="Warren, Raj" uniqKey="Warren R">RAJ Warren</name></author><author><name sortKey="Storch De Gracia, I" uniqKey="Storch De Gracia I">I Storch de Gracia</name></author><author><name sortKey="Garcia, A" uniqKey="Garcia A">Á García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michaelis, L" uniqKey="Michaelis L">L Michaelis</name></author><author><name sortKey="Menten, Ml" uniqKey="Menten M">ML Menten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uhland, K" uniqKey="Uhland K">K Uhland</name></author><author><name sortKey="Mondigler, M" uniqKey="Mondigler M">M Mondigler</name></author><author><name sortKey="Spiess, C" uniqKey="Spiess C">C Spiess</name></author><author><name sortKey="Prinz, W" uniqKey="Prinz W">W 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Aeschbacher</name></author><author><name sortKey="Wiemken, A" uniqKey="Wiemken A">A Wiemken</name></author><author><name sortKey="Boller, T" uniqKey="Boller T">T Boller</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Alexandrino, Andre Vessoni" sort="Alexandrino, Andre Vessoni" uniqKey="Alexandrino A" first="André Vessoni" last="Alexandrino">André Vessoni Alexandrino</name><name sortKey="Goto, Leandro Seiji" sort="Goto, Leandro Seiji" uniqKey="Goto L" first="Leandro Seiji" last="Goto">Leandro Seiji Goto</name><name sortKey="Novo Mansur, Maria Teresa Marques" sort="Novo Mansur, Maria Teresa Marques" uniqKey="Novo Mansur M" first="Maria Teresa Marques" last="Novo-Mansur">Maria Teresa Marques Novo-Mansur</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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